Abstract: Ecological changeover of industry development is the basic requirement and working focus for the realization of the concept of scientific development, as well as a difficult task. In accordance with the idea of system engineering, the construction of Eco-Industrial chain is the effective means to resolve this problem. The difference between two industrial chains was analyzed from the aspects of goal -orientation, paths to construction of Eco -industrial chain and bases of inter -enterprise collaboration by comparing the Eco-industrial chain and the traditional one. The principles of building up the Eco-industrial chain were brought out by analysis of its necessity and feasibility, including the principles of mutual benefits, the matching of supply and demand, the efficiency of scale economy, the systematic guide and technology promotion. The case study of seawater resource comprehensive utilization in Cao Feidian industrial park was made to prove the application and values of these principles in Eco-industrial chain construction.